FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030339
Sequences flagged as poor quality0
Sequence length36-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC272431.3417956311729224Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC245411.2087144068059572Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG157740.776914594065326Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC116660.5745838502831301Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT110180.5426679978072627TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT84870.4180090122880957Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA59460.2928574981813382Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCA31750.1563778265599981TruSeq Adapter, Index 1 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG26030.12820519134981892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGA23710.11677852811771826TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)