FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030339
Sequences flagged as poor quality0
Sequence length36-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG272731.343273216935694Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA253731.2496927852934905Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG153410.7555881062226554Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG114930.5660631057178136Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG107810.5309950702813668Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA73920.36407713194693103Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG46590.22946906895843505Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTC33580.1653910997129051Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCAC24820.12224559543997332Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)