FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289945
Sequences flagged as poor quality0
Sequence length36-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC87710.38302229966221896Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC76360.3334577904709502Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG51290.22397917853922258Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC36930.16127024884877147Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG31340.13685918220743293TruSeq Adapter, Index 2 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)