FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594932
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC494631.3759091966134547Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC433601.20614242494712Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT248860.6922523152037368TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG187780.5223464588481784Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTA126260.35121665722745243TruSeq Adapter, Index 18 (97% over 37bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC85460.2377235508209891Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC80490.22389853271216256TruSeq Adapter, Index 18 (100% over 37bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT44160.12283959752228972Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGA43620.12133748287867475Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)