FastQCFastQC Report
Wed 8 May 2024
EGAF00006469375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594932
Total Bases523 Mbp
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA536041.491099136228446Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG457011.2712618764416128Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG269340.7492214039097262Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG194000.5396485941875951Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCAC137870.383512122065174Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTC88420.24595736442302665Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG85910.23897531302400155Illumina Single End PCR Primer 1 (100% over 28bp)
GGAGTTCAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG42640.11861142296989205Illumina Multiplexing Index Sequencing Primer (100% over 27bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA41620.11577409530973048Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)