FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3594932
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA534921.4879836391898371Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG455671.2675344067704202Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG268840.7478305570174901Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG193070.5370616189680361Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCAC137620.38281669861905593Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTC88090.24503940547415085Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG85520.23789045244805743Illumina Single End PCR Primer 1 (100% over 28bp)
GGAGTTCAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG42460.11811071808868709Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA41280.11482831942300994Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)