FastQCFastQC Report
Thu 21 Jul 2022
EGAF00006469385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011114
Sequences flagged as poor quality0
Sequence length36-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA52450.2608007303414923Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG51830.25771786184174544Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG24320.12092800308684638Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG20860.10372360791083947Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)