FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658644
Sequences flagged as poor quality0
Sequence length36-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC577741.2401462743236014Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC502221.0780390173621337Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG397980.8542829200943451Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC301030.6461751531132235Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT217250.4663374149215952TruSeq Adapter, Index 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT191690.41147166428686116Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA110930.23811649913580002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTA73940.15871571212567434TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGG57020.12239613071958277TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)