FastQCFastQC Report
Tue 26 Jul 2022
EGAF00006469391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006469391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658644
Sequences flagged as poor quality0
Sequence length36-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG576301.2370552461188278Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA513871.103046294157699Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG384070.8244244462551764Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG278840.5985432670966058Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG223540.47983919784383605Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA167410.35935349427859264Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG88710.19042021669824954Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTC77110.16552026727090544Illumina Single End PCR Primer 1 (97% over 38bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCAC58630.125852071976309Illumina Single End PCR Primer 1 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)