FastQCFastQC Report
Mon 8 Aug 2022
EGAF00006481047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006481047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26773019
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG3071091.1470839355098505No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGTT2514110.9390461344684363TruSeq Adapter, Index 2 (97% over 36bp)
GTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCAC2252300.8412573867743491No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA1738160.6492207696113763No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTT1460970.5456874325603698No Hit
GTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCC1400920.5232581353638154No Hit
GTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGC1133800.423486047651182No Hit
CTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAA1121740.4189815126938057No Hit
TGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACC1089750.4070329162355579No Hit
GCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAG942290.35195507835705786No Hit
GCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGT941880.35180193910892155No Hit
GTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCT933970.3488474721509741No Hit
GAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGT928650.3468603970288147No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA897670.3352890460354882No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGTT826010.3085232935441461TruSeq Adapter, Index 2 (97% over 36bp)
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT823200.30747372942887014No Hit
GGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGG819240.3059946283980899No Hit
CGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTC797040.29770269837704894No Hit
GGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGT785250.29329901121722585No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG768840.28716970618815907No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC764860.28568313495015263No Hit
GGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCA763230.28507431306122033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGT763090.2850220216106372TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTTT762210.284693332492686TruSeq Adapter, Index 2 (97% over 36bp)
GCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCC733180.27385032670391035No Hit
GGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGG732110.27345067061731065No Hit
GCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACG725560.27100417775074226No Hit
ATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAG720900.2692636194670463No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA704170.26301479112236087No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA692980.2588352101793227No Hit
GGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGA680260.254084158383483No Hit
GGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATC677680.253120501651308No Hit
CGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGG674160.2518057451795033No Hit
TTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAG669140.24993072316573636No Hit
CGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCG655970.24501159170730805No Hit
GGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCT655630.24488459818446323No Hit
GCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGG648800.2423335224167286No Hit
GCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACG644700.24080212993536515No Hit
GGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGG637620.2381576765773034No Hit
GGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGC635660.23742559626913945No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT621200.2320246364446236No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGAT619530.231400874141239TruSeq Adapter, Index 2 (97% over 36bp)
CTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCA597300.2230977388093588No Hit
CTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGG574290.21450326539565823No Hit
GTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGT570080.2129307867745509No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT555830.20760826412590974No Hit
GGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGG550080.20546057954838787No Hit
CGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC539620.2015536611691046No Hit
CTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGG536800.20050036195021562No Hit
GGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCG531600.19855810807141325No Hit
GCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTC528360.1973479345007748No Hit
GCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGG528200.19728817284296551No Hit
GCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTC525540.19629463528188584No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTC516250.19282472402533313No Hit
CTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGA514090.1920179416449075No Hit
TTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGG502710.18776739373322077No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT497380.1857765835074483No Hit
TGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGAC493390.1842862771658288No Hit
GGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGG474280.17714849416123002No Hit
GCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCG462650.1728045686592162No Hit
GGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTC460680.17206875324743914No Hit
CGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGG458190.17113871244778184No Hit
TGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAAC458090.17110136141165105No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG456180.17038795662155248No Hit
GGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACC454250.16966708162422772No Hit
CAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGCGC440410.16449769822372293No Hit
CACGGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAA427720.15975785173872248No Hit
GGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAA427510.15967941456284776No Hit
CAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGA425130.15879045990293436No Hit
CAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCA414740.1549096872489427No Hit
GGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGC414550.15483872028029413No Hit
GGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAG410720.1534081755964839No Hit
CGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAG399460.14920244892815412No Hit
GCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGG396890.14824252729959217No Hit
GCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATG394810.14746562574807123No Hit
CAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCG392200.14649076370505695No Hit
GGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGA381590.14252781877157747No Hit
AGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTG380200.14200863936935912No Hit
CCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAA367140.13713059405067465No Hit
GGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCG362420.13536762514530018No Hit
TCCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGA360530.1346616905624278No Hit
CCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTC360360.1345981938010054No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGT359150.13414624626382254TruSeq Adapter, Index 2 (97% over 36bp)
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA347070.1296342410992201No Hit
TGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCAC345250.12895445224163923No Hit
GGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGA343850.12843153773580782No Hit
GTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCG343280.12821863682986218No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG341330.12749029162531128No Hit
GGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCG338720.12651542958229703No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA336620.1257310578235499No Hit
AGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAG330270.12335926702924313No Hit
AGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTC329580.12310154487994052No Hit
GCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTG329420.12304178322213119No Hit
ACCGGGTCCACCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAG328860.12283261741979865No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA327960.1224964580946213No Hit
GTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCGCCACGG327660.12238440498622885No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT317730.11867544709843893No Hit
GGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAACGG316310.11814506238538133No Hit
TGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCG314610.1175100947711575No Hit
GCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTG313440.11707308764842696No Hit
GTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCC311800.11646053065588159No Hit
GCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCT307970.11502998597207137No Hit
GCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGG306500.11448092574094838No Hit
CTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCT301970.11278892380422245No Hit
GGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCG301330.11254987717298523No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCC299100.11171694906726805No Hit
GCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCAC298710.11157128002635787No Hit
GAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGG297010.11093631241213403No Hit
CGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGGG296690.1108167890965154No Hit
GGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGG293600.10966264208007322No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC293560.10964770166562089No Hit
GTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGT292950.10941986034522294No Hit
CGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTG292750.10934515827296129No Hit
CGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGC289640.10818354104929295No Hit
GGAGGAACGGCTACCGAGGCTCCAGCTTAACGGTATTTGGAGGTCAGCAC289030.10795569972889497No Hit
GACAGGAACATCCTCTCCAGGGCCTCCGCACCATACTCGCCAGCGTGCGC287880.10752616281339061No Hit
GCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCCGTGGCTCAGGTC283630.10593874377783097No Hit
GTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCAGAGCC281720.1052253389877324No Hit
AGCGCGTTGGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTC280930.10493026580229894No Hit
GTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCACGTGCG279110.10425047694471812No Hit
CAGGAAGGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGC278380.10397781438096317No Hit
CAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACA274040.10235677941288579No Hit
CGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGG273030.10197953394796455No Hit
CTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCAT271820.10152758641078169No Hit
TTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGC271290.10132962591928836No Hit
GCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCG270080.1008776783821055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)