FastQCFastQC Report
Tue 13 Sep 2022
EGAF00006566420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006566420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21413004
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGCTTAA1259850.5883574299056779TruSeq Adapter, Index 1 (97% over 36bp)
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG838580.39162183876676065No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGT555560.25944981843743176No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAA512900.23952734515904445No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC424780.19837478197827826No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGT419130.19573619843343792No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTC382120.17845230869989098No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCA377880.17647220352641788No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAAC367630.1716853926707341No Hit
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAG362530.16930366239132072No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAA357600.1670013231212211No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT348270.16264415772770602No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACA339780.15867927732138845No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAA331460.15479378792438464No Hit
CCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCCCCGC331050.15460231549015727No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAA322340.15053469377766893No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAA321430.15010971837487164No Hit
GCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCT317310.14818565391385535No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGG292550.1366225869102719No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCC289270.1350908074364531No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTT284680.13294725018498105No Hit
CACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCT280340.13092044441779396No Hit
CACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC279130.1303553672338547No Hit
CCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGC250250.11686823576925497No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGG249870.11669077351314182No Hit
CCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGA248840.11620975739788775No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGA230970.1078643613011981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)