FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006598708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006598708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58975274
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2070110.35101320597510066TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1288330.2184525670876917No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT690410.11706770535733332No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG666790.11306263706379728No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT619910.10511354300787142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)