FastQCFastQC Report
Tue 13 Sep 2022
EGAF00006598938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006598938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68534155
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1542360.2250498309930282TruSeq Adapter, Index 4 (100% over 50bp)
CCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTG1314610.19181822552565211No Hit
CTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGATAATATA1115350.16274367138545737No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG943900.13772694797214616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG942000.1374497139419024No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT912250.13310881267887523No Hit
GGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTT908850.13261270967738642No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGTTAAAAAATAGTAGAGGGATGATGCT848710.12383752305693417No Hit
CCCGGATTTGTTCACTGGGTCTTTGTCTTTCTTGGCCGACTTTCCAGCGT842180.12288471346878065No Hit
CCGGATTTGTTCACTGGGTCTTTGTCTTTCTTGGCCGACTTTCCAGCGTC820130.11966733959147816No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG772500.11271752019120977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)