FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11493135
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGG2544972.2143392555643No Hit
CAGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAA1638021.4252160093829926No Hit
CACCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT1540491.340356656386617No Hit
CACTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC1185941.03186815433735No Hit
CACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAG1050770.9142588162411736No Hit
CAGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGA1011610.8801863025188514No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940360.818192773338171No Hit
CACTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTAT849300.7389628678337112No Hit
CACTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAGC682720.5940241718208304No Hit
CACCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTG633480.5511812051281048No Hit
CACCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT601290.5231731812077384No Hit
CAGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA549930.4784856351204437No Hit
CACCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC524750.4565769043868361No Hit
CATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGC421000.36630562505356457No Hit
CACACTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCT406590.35376770567821575No Hit
CAGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT363840.31657158816980746No Hit
CAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGG331040.28803281263119246No Hit
CAGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG314450.2735981087840698No Hit
CATGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA309860.2696044203779039No Hit
CAGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCT297430.25878926855031287No Hit
CACCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT287830.2504364561975475No Hit
CATGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATG285140.24809592856953303No Hit
CATTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT262980.2288148533885663No Hit
CAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAG255320.2221500051987556No Hit
CAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA254290.22125381804007346No Hit
CAGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGT247710.2155286612399489No Hit
CAGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGA238910.2078719165832473No Hit
CACCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCC228500.19881433568821735No Hit
CAAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG226360.19695235460124674No Hit
CAGCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC202430.17613122964273892No Hit
CACTGCACTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAG194580.16930106537511305No Hit
CATGTAGCCAGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGC192650.1676218020583592No Hit
CACTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT187720.16333228488136614No Hit
CACCAGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTG185160.16110486825396203No Hit
CAGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAG184080.16016517686427595No Hit
CACTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTC173550.15100318581483643No Hit
CACTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC172410.15001128934794555No Hit
CAGCCTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGT172240.149863374962532No Hit
CACCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT170720.1485408463400108No Hit
CATGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGTG169380.14737493294910395No Hit
CACTGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGT160260.13943976121397686No Hit
CACCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC152480.1326705028697566No Hit
CAGCCAGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGT150810.13121746155422345No Hit
CAGCTGCACTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGA148870.1295294973912688No Hit
CACAGAAAATGCCCTGCAGTCTGCAGTGTTCTACTGTGAACTGCTTGTGT143350.1247266302884287No Hit
CAAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATC138810.12077644611326675No Hit
CATTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCC136040.11836631171564589No Hit
CAAGGAACTTGAGGCTATGTAGCCTGAAAATGCCCTGCAGTCTGCAGTGT132920.11565164770099717No Hit
CACTCAGTTCCTGTTCTAGGAACTTGAGGCTATGTAGCCTGAAAATGCCC131830.11470325546511027No Hit
CATCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC124670.10847344958533943No Hit
CAAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA123780.10769907427346846No Hit
CACGCAAACCCAAGAGGCGGAGGGCCGCTGACGTGGAACCATCATCACCC122500.1065853659597664No Hit
CAGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG120600.10493220518161493No Hit
CACTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGG117050.10184340478033192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)