FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977571
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT1251952.5151826061345988No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC950311.9091842185676506No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA523091.0508941007571766No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT368320.7399593094704224No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT345810.6947364487618559No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA281210.5649542718727669No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA272450.5473553265237201No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT270160.5427546889838437No Hit
GGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCC190950.3836208463927486No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG190360.38243552929732194No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC181030.3636914470933715No Hit
GGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCG172840.3472376385992284No Hit
CTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC168780.3390810497730721No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT165810.3331142840554158No Hit
GGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAG145920.2931550348553541No Hit
CTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGC144640.2905834994618861No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC136510.27425023168931195No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTT134090.26938842258603646No Hit
GGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG133240.26768076236381155No Hit
GCCGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCG129090.259343362455302No Hit
GGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCC124210.24953938376770518No Hit
GGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGC121810.24471775490495262No Hit
GGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCG121000.24309045516377364No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCC116920.23489368609709438No Hit
GCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAA114590.23021268807617207No Hit
CACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCC112860.226737097270938No Hit
CTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCC108420.2178170838748458No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG108020.21701347906438706No Hit
CTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGC105980.21291509453104737No Hit
TTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC103010.20694832881339112No Hit
CTCCTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTCATCATCCTCTTCTT98670.1982292166199136No Hit
CTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC95830.19252362246565646No Hit
CCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT95080.19101686344604626No Hit
GTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGG94790.19043424995846367No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA88660.17811900623818322No Hit
CGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACG85210.17118791474797646No Hit
GCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC85100.1709669234251003No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTC84810.17038430993751771No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC83310.1673707918982974No Hit
GGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGA83250.16725025117672856No Hit
TGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGG82660.1660649340813019No Hit
CTTCATTCCAGGGAGGCCAGCTGTTCCGGGCAATCCTCGAGCACCCTGAG78830.1583704180211593No Hit
ATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA75230.1511379747270305No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG74870.15041473039761763No Hit
GCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT74090.14884770101722306No Hit
GAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA72550.14575382249695684No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCT71510.14366444998976408No Hit
CTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAA70710.14205724036884657No Hit
GGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT70180.14099246399498871No Hit
CGCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAG69630.13988750738060793No Hit
GGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGC69460.13954597533616295No Hit
CTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCC68980.13858164956361246No Hit
CTCCTTTTCTCGTTCACGTTCTCTTTCCCTTTCTCGTTCCCGTTCTCGCC68460.13753696331001605No Hit
CTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTCTT67040.13468416623288748No Hit
CCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT66400.13339839853615348No Hit
GGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAG65440.13146974699105246No Hit
CTCTTCTTCTTCTTCCTCATCTTCTTCTTCCCCTTCATCTTCCTCCTCTC64780.13014379905379553No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA62750.12606550464071734No Hit
AGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAA61430.12341360876620344No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA61290.12313234708254286No Hit
CAGGAAAACACGGCCGCGGGATCCCACCGCCACAGACACGAGGGCGGTCC60650.12184657938580885No Hit
GGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGA57460.1154378310224003No Hit
CCCGTTCTCGTTCCCGCTCCCGTTCTTTCTCCTTTTCTCGTTCACGTTCT57230.11497575825638649No Hit
CTCCGGAGCCCTCCTCCAGCCCTTCCTCCAGCCCTTCCTCCGGAGCCCTC55770.11204260069821205No Hit
GCCGCCACCGCCGCCGCCGCCGCCGCCCCGACCCGCGCGCCCTCCCGAGG53280.1070401607531063No Hit
CCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCC53080.10663835834787691No Hit
GAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGA53070.10661826822761543No Hit
CTCATCTTCTTCTTCCCCTTCATCTTCCTCCTCTCCATCCTCGGCAGTTT52740.10595529425898698No Hit
GAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAA52570.105613762214542No Hit
AGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAA52080.10462934632173003No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)