FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4977571
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT905951.8200644450877748No Hit
TTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGG521741.0481819345218781No Hit
TTCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC514841.0343197515414646No Hit
TTGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGG460360.9248687763569822No Hit
TTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAG430540.8649600377372819No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA429550.8629711158313964No Hit
TTCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT379640.7627013256064052No Hit
TTGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAA363680.7306374936691009No Hit
TTGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCG265240.5328703498152011No Hit
TTATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAG251490.5052464344556813No Hit
TTTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA242410.48700460525826755No Hit
TTAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATC206700.41526278580456216No Hit
TTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCC194800.3913555426934141No Hit
TTGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGA192370.38647364346987717No Hit
TTGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGA186360.37439948119273436No Hit
TTGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCG185550.3727721814515554No Hit
TTGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGA183490.3686336166776928No Hit
TTGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA181740.3651178456319357No Hit
TTGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACC181140.3639124384162476No Hit
TTCGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAA176290.35416873008943517No Hit
TTGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA161630.32471661378612177No Hit
TTGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGG157210.31583678063055254No Hit
TTCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT153180.3077404621651806No Hit
TTCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTT151430.3042246911194235No Hit
TTGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGC134370.26995094595335756No Hit
TTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAA123260.24763082234286563No Hit
TTGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG120760.2426082922774984No Hit
TTGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCG119500.2400769371245533No Hit
TTTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT109280.2195448342173321No Hit
TTCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCGG108250.21747555183040082No Hit
TTGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGCG99790.20047931008919814No Hit
TTCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC96370.19360848895977578No Hit
TTCTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCC96070.1930057853519317No Hit
TTGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCC94810.1904744301989866No Hit
TTCGCCGCGGGACCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGT94020.1888873106983306No Hit
TTCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAAC92630.1860947839819864No Hit
TTGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCG90530.18187585872707793No Hit
TTCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCGGCGGCGGC85790.17235314172314167No Hit
TTGCGAAAGAAAGAAGTGGTGGAGGAGGAGGAGAACGGGGCTGAGGAGGA85670.17211206028000403No Hit
TTAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGG85450.1716700776342517No Hit
TTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGG83550.16785295478457263No Hit
TTCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGA82790.166326105644701No Hit
TTCCGCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGG79100.158912851268219No Hit
TTCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGCC78270.15724537128651705No Hit
TTCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCG77830.15636140599501244No Hit
TTCGGGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCG77280.15525644938063163No Hit
TTAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAA76990.15467383589304903No Hit
TTCCCGGCCCCGTCCGCCCCCCGTTCCCCCCTCCTCCTCGGCGCGCGGCG74880.1504348205178791No Hit
TTCTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCC74770.15021382919500292No Hit
TTCGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAG74130.14892806149826893No Hit
TTAGCGAAAGAAAGAAGTGGTGGAGGAGGAGGAGAACGGGGCTGAGGAGG70910.14245904277407595No Hit
TTGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAA66750.13410155274530489No Hit
TTCTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTC66570.13373993058059844No Hit
TTCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGGC66040.1326751542067406No Hit
TTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAG65200.1309875841047772No Hit
TTCGCGGGTCGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGG63690.1279539759452954No Hit
TTCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCT63170.126909289691699No Hit
TTGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCC62980.1265275774067311No Hit
TTCCTGGATTGGCTGGACCCCCTGGTGAATCTGGACGTGAGGGGGCTCCT62230.12502081838712095No Hit
TTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCC61200.12295153600018965No Hit
TTGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAA60430.12140459674005655No Hit
TTCAAAGGTCCCCGTGGTGAGACTGGCCCTGCTGGACGTCCTGGTGAAGT60430.12140459674005655No Hit
TTCCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC60270.12108315481587305No Hit
TTGGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGA59780.12009873892306108No Hit
TTCTTGATGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGG59770.1200786488027996No Hit
TTAAAACATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGG58440.11740666280802424No Hit
TTGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGA57930.11638206667468932No Hit
TTCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAA57930.11638206667468932No Hit
TTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56900.11431278428775803No Hit
TTCTCGACGTGGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGG56860.11423242380671214No Hit
TTGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACC56530.11356944983808369No Hit
TTTGAAGATGAAGAAGAGGATGATGAGGACGAGGATGACGACGACGACGA56520.1135493597178222No Hit
TTCATGTTGGCTTTTCTCTTGACGTGGGAGAAATTGAAAAGAAGGGGAAG55920.11234395250213408No Hit
TTGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGG55290.11107827492566154No Hit
TTAGCTGGGGCTGCTGGTCCTGCTGGTCCTGCTGGTCCTCGGGGAAGCCC55040.11057602191912481No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT54940.11037512071651014No Hit
TTCTCCTTTTCTCGTTCACGTTCTCTTTCCCTTTCTCGTTCCCGTTCTCG53860.10820538772827147No Hit
TTGGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGAC53800.10808484700670266No Hit
TTGGAGAACGGGGCTGAGGAGGAAGAAGAAGAAACTGCCGAGGATGGAGA53490.10746205327859712No Hit
TTCCCTCGTGTCTGTGGCGGTGGGATCCCGCGGCCGTGTTTTCCTGGTGG52310.1050914190877438No Hit
TTGGAAGGGCTGGAGGAGGGCTCCGGAGGAAGGGCTGGAGGAAGGGCTGG51080.10262033429558313No Hit
TTGCGGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGGACCG50890.10223862201061522No Hit
TTTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCC49990.10043051118708302No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)