FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18860170
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC5206942.760812866479995No Hit
GCTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGT1165450.6179424681749953No Hit
GCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGA1109470.5882608693346878No Hit
GCCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGT1029840.5460396168221178No Hit
GCTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTC1018800.5401860110486809No Hit
GCAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA902400.4784686458287492No Hit
GCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGT758010.4019104811886638No Hit
GCGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTAC708470.3756434857161945No Hit
GCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACT621750.32966298819151685No Hit
GCCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCT620700.3291062593815432No Hit
GCCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTC609920.32339051026581417No Hit
GCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACT602970.3197054957617031No Hit
GAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGG601700.3190321190105922No Hit
GCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCC599630.3179345679280728No Hit
GGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCC561540.29773856757388717No Hit
GCCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTA534430.2833643599182828No Hit
GCGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCC533600.28292427904944656No Hit
GCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGA518510.27492329072325433No Hit
GCCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCC508940.2698491052837806No Hit
GCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGC501090.26568689465683504No Hit
GGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGA497190.2636190447912187No Hit
GCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCA496210.2630994312352434No Hit
GCGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTG483190.2561959939915706No Hit
GGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGC464030.24603701875433784No Hit
GCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT463560.24578781633463534No Hit
GCTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCT461330.24460543038583427No Hit
GGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGT453530.24046973065460173No Hit
GCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCT451850.23957896455864394No Hit
GCGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGCACGTGGAC451110.23918660330209113No Hit
GCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCG449290.23822160669813688No Hit
GCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAA446700.23684834230020194No Hit
GCTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT443130.2349554643462917No Hit
GGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCC408200.21643495260116954No Hit
GCCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCAC405360.2149291337246695No Hit
GCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCC403120.2137414455967258No Hit
GCACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTT397360.21068739041058485No Hit
GCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTG393250.20850819478297386No Hit
GCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCC384260.20374153573377124No Hit
GCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC379920.2014403899858803No Hit
GCCCTGAGCGACCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTT369910.19613290866413186No Hit
GCCTCCCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTC354290.18785090484338157No Hit
GCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGG348690.18488168452352233No Hit
GCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGA342540.18162084435081974No Hit
GGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAG338830.17965373588891298No Hit
GCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA334080.17713520079617523No Hit
GACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCC333860.1770185528550379No Hit
GGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGC329100.17449471558315752No Hit
GCCGCCGAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGC325870.17278211172009583No Hit
GACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC320160.16975456742966794No Hit
GGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAA316920.16803666138746365No Hit
GGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTG313350.16614378343355335No Hit
GCAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAA308670.16366236359481384No Hit
GCTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTG306840.16269206481171697No Hit
GGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCT304420.16140893745920634No Hit
GAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTT303680.16101657620265353No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA296490.15720430939911995No Hit
GCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACC288500.15296786826417788No Hit
GCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGG285660.15146204938767785No Hit
GCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTA283030.15006757627317252No Hit
GAGTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGT281350.14917681017721474No Hit
GCCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG276110.14639846830648928No Hit
GCAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTG275020.14582053077994525No Hit
GCCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTG273020.14476009495142408No Hit
GCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA272190.1443200140825878No Hit
GACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC269740.14302098019264936No Hit
GCATGCCCAACGCGCTGTCCGCCCTGAGCGACCTGCACGCGCACAAGCTT262270.13906025237312283No Hit
GCGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGT255340.135385842227297No Hit
GCCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGT250320.13272414829770887No Hit
GCCTGCACGCGCACAAGCTTCGGGTGGACCCGGTCAACTTCAAGCTCCTA250200.13266052214799762No Hit
GCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCCTG241520.12805823065221578No Hit
GCACGGCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGC238950.12669557061256606No Hit
GCTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTT235490.12486101662922446No Hit
GCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCG229700.12179105490565567No Hit
GGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGC229050.1214464132613863No Hit
GCGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTG224870.11923010237977706No Hit
GCCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGCCCAGGTTAAGGG224860.11922480020063447No Hit
GCAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGC222800.11813255129725765No Hit
GGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAG221290.11733192224672419No Hit
GCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCT221180.11727359827615551No Hit
GGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGT220720.11702969803559565No Hit
GGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGG218560.11588442734079278No Hit
GGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGG218040.11560871402537728No Hit
GGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAG216270.11467022831713605No Hit
GCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCTGCGG215040.11401806028259553No Hit
GCTCAAACAGACACCATGGTGCATCTGACTCCTGAGGAGAAGTCTGCCGT214400.11367872081746877No Hit
GCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC210420.11156845351871164No Hit
GACCCGGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGC209700.11118669662044404No Hit
GCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTAT209620.11114427918730319No Hit
GGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTG208930.11077842882646338No Hit
GCGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCG208210.11039667192819576No Hit
GGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCATCTGACTCCTGA206380.1094263731450989No Hit
GCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAGCCACGGCTCTGC205120.10875829857313057No Hit
GGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGG203230.10775618671517806No Hit
GTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCAT196040.10394391991164448No Hit
GGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA195770.10380076107479412No Hit
GCTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTCACCCCT195610.10371592620851244No Hit
GCTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAAC194110.10292059933712157No Hit
GAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCTCCCCGCCGAGTTC192990.1023267552731497No Hit
GCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGTAAGGTCGGCGC191740.10166398288032398No Hit
GCGCTGACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTC191260.10140947828147891No Hit
GGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATACCGT190020.10075200806779579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)