FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31228378
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC8219272.6319874826672076TruSeq Adapter, Index 5 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1647470.5275554177037308TruSeq Adapter, Index 5 (100% over 50bp)
CTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCT654930.20972270798054257No Hit
CTTCCATCCAGGACTGGGTTCAGAAGACCATAGCTGAGAACTAATGCAAG485550.15548357971073618No Hit
CTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCAAG425570.13627669038718565No Hit
CTGAGAACTAATGCAAGGCTGGCCGGAAGCCCTTGCCTGAAAGCAAGATT404340.1294783866136115No Hit
ATCAGCTTCAAACCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCC383740.12288182242446277No Hit
GTGAAGGTGACTTCCATCCAGGACTGGGTTCAGAAGACCATAGCTGAGAA368470.11799203916386562No Hit
CTTTGATAAGAGCTGTGCTGTGGCTGAGTATGGTGTGTATGTGAAGGTGA358590.11482825012557489No Hit
CCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCT355710.11390601202534438No Hit
ATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATC342690.10973672728055234No Hit
GTTCAGAAGACCATAGCTGAGAACTAATGCAAGGCTGGCCGGAAGCCCTT333730.10686754207983522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)