FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31228378
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC7532142.4119536403715878Illumina Single End PCR Primer 1 (97% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1047300.33536804249007107Illumina Single End PCR Primer 1 (98% over 50bp)
ATCTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTG602680.19299113133573573No Hit
ATCTTCCATCCAGGACTGGGTTCAGAAGACCATAGCTGAGAACTAATGCA493850.15814141868015047No Hit
ATCTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCTTTCAGGCA394490.12632420422219814No Hit
ATCTGAGAACTAATGCAAGGCTGGCCGGAAGCCCTTGCCTGAAAGCAAGA387450.1240698444216347No Hit
ATCTTTGATAAGAGCTGTGCTGTGGCTGAGTATGGTGTGTATGTGAAGGT372440.11926331876730836No Hit
ATGTGAAGGTGACTTCCATCCAGGACTGGGTTCAGAAGACCATAGCTGAG368070.11786395053883363No Hit
ATATCAGCTTCAAACCACATCTTATCGCATCCACTCCTGTCCACTCCCGT367230.1175949644262664No Hit
ATCCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCT325670.10428655628544012No Hit
ATGTTCAGAAGACCATAGCTGAGAACTAATGCAAGGCTGGCCGGAAGCCC323120.10346999130086104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)