FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355238
Sequences flagged as poor quality0
Sequence length100
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA518563.8263389899043565No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTC116700.8611033633944739No Hit
CTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTT110300.8138791857961479No Hit
CACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCC68250.5036015814196473No Hit
GCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC65270.48161282372542685No Hit
GGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCG65230.48131767261543723No Hit
GGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAG60500.4464160538591746No Hit
GGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCC57710.4258292639374044No Hit
GGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAA56440.41645821619523654No Hit
GGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGA53940.39801127182089047No Hit
CCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCT51930.38317992854391625No Hit
TTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTT45800.33794802093801973No Hit
GGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGC44840.33086439429827086No Hit
CTCGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGC41260.30444836995420727No Hit
GGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGCCGCC40780.30090655663433286No Hit
GCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAA40280.29721716775946366No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG36760.2712438700803844No Hit
GGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAG36210.26718554231802827No Hit
CTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGC36150.266742815653044No Hit
GAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCA34620.25545328569594417No Hit
CCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTT34010.2509522312686037No Hit
GGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCG33270.24549193573379732No Hit
GGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGG32840.2423190613014098No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC32060.2365636146566138No Hit
CGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCC30630.22601196247448788No Hit
AGGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGG29610.21848560916975468No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC28350.20918834920508428No Hit
GTCGGGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACC27260.20114548145786937No Hit
GGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCC27030.19944836257542956No Hit
GCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACC26030.19206958482569114No Hit
GGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGAT25950.19147928260571206No Hit
GCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCC25340.18697822817837162No Hit
GGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCC24230.17878778487616198No Hit
CCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCC24100.17782854376869597No Hit
CGGAGAGAAAGAGAGAGGGAGCGTGAAAGGGAACGAGAAAGGCGAGAACG24090.17775475599119858No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC23540.17369642822884246No Hit
GCCGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGGG23370.1724420360113869No Hit
GGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCC22980.16956431268898894No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC22130.1632923516017113No Hit
CTCCTCTTCGGCAGCTCTCTTCAGCGCGGGCCCTTCATCATCCTCTTCTT21660.15982432605933422No Hit
CTCTTCTTCTTCTTCCTCATCTTCTTCTTCCCCTTCATCTTCCTCCTCTC21560.1590864482843604No Hit
CCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTCAGGCACGGCCG20720.1528882749745801No Hit
GGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAAC20310.14986297609718735No Hit
GGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGA20210.14912509832221352No Hit
GGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGA20150.14868237165722922No Hit
CGCGGGTCGGGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGACCG19670.14514055833735476No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19660.1450667705598574No Hit
GGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGCCGC19080.1407870794650091No Hit
GGCGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGA18900.13945889947005619No Hit
CTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTC18770.13849965836259018No Hit
CGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGA18600.13724526614513466No Hit
GCCGCCGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGG18560.13695011503514512No Hit
CTCCGCCGCCTGCCGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGG18150.13392481615775237No Hit
CTCCGCCGCCTGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGG17940.13237527283030728No Hit
GCCAGGGGCACCAGGAGCACCAGGAGCACCAGGGGGTCCAGCGGGGCCGG17870.1318587583878256No Hit
GCCCTTTCTTTTTCTCTTTCTTCCTCTTCCCTTCGTCTTTTCCTTTCTCT17550.12949754950790932No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC17420.1285383084004433No Hit
CGGCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGCGGCGGCGGGACCGAAA17360.128095581735459No Hit
GGGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGACCGAAACCCCC17270.12743149173798254No Hit
CCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCG17130.12639846285301917No Hit
AAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAA17120.1263246750755218No Hit
CAGGAAAACACGGCCGCGGGATCCCACCGCCACAGACACGAGGGCGGTCC17080.12602952396553224No Hit
AAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGGG17010.12551300952305056No Hit
CCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTT16850.12433240508309241No Hit
CTCCTCTTTCTCCTCCTCCTCCTCTTCCTCAAACTCGGGTTCCAGCTGGG16810.12403725397310288No Hit
TTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTCTTTTC16580.12234013509066304No Hit
CCCTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTC16580.12234013509066304No Hit
CCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCT16450.12138089398319706No Hit
CCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCC16150.11916726065827551No Hit
GGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGCCACAGACAC16040.11835559510580429No Hit
CTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCACCGCCGC15880.11717499066584616No Hit
CTTCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCC15820.11673226400086185No Hit
GCCGCCGCCGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAA15730.11606817400338539No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG15690.11577302289339585No Hit
CTCCTTTCTCTCCTTTCCTCCCAGGTTCTCCTTTCAAGCCAAAGTCGCCT15230.11237878512851618No Hit
CGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGA14960.1103865151360868No Hit
GGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGG14920.11009136402609725No Hit
GCCAGCAGGGCCAACGGGGCCAGGGGCACCAGGAGCACCAGGAGCACCAG14910.11001757624859987No Hit
CTCCTTTTCTCGTTCACGTTCTCTTTCCCTTTCTCGTTCCCGTTCTCGCC14870.10972242513861034No Hit
GGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGCCACA14830.1094272740286208No Hit
GCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGC14740.10876318403114434No Hit
TCCCCTTCTTTTCTTCCCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCT14630.10795151847867311No Hit
CGCCGCCGCCGCCGCCGCCGCCGCGCGCCGAGGAGGAGGGGGGAACGGGG14630.10795151847867311No Hit
GGGAGAAATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGAT14450.1066233384837202No Hit
GAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAAGGAAAGAAGAAGGG14430.10647576292872545No Hit
CGGGTCGGGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGCGGGACCGAA14340.10581167293124898No Hit
CTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCTT14260.10522137071126991No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.10485243182378298No Hit
CTTCTTTTCTTCCCCTTCTTCTTTTCTTCTTTGATCTTCTTCCCCTTCTT14120.10418834182630651No Hit
ATTGAAAAGAAGGGGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAGAA14090.10396697849381438No Hit
AGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGCCCGC13860.10226985961137454No Hit
GGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCC13810.10190092072388762No Hit
CGGGCCACCAGGAAAACACGGCCGCGGGATCCCACCGCCACAGACACGAG13760.10153198183640069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)