FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7928428
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC383981248.43093738128164Illumina Single End PCR Primer 1 (97% over 49bp)
ACGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC775830.9785420262377359Illumina Single End PCR Primer 1 (97% over 49bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC518330.6537613761517416Illumina Single End PCR Primer 1 (100% over 49bp)
ATGATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC155030.19553687061293867Illumina Single End PCR Primer 1 (100% over 34bp)
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGC147810.18643039956975077Illumina Single End PCR Primer 1 (97% over 45bp)
ATGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC131010.16524082705928592Illumina Single End PCR Primer 1 (100% over 31bp)
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGC100890.1272509506298096Illumina Single End PCR Primer 1 (97% over 42bp)
ATGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGC90990.11476423825757137Illumina Single End PCR Primer 1 (100% over 29bp)
ATGATCGGAAGAGCGCCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC80800.10191175350271202Illumina Single End PCR Primer 1 (100% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)