FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077797
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC66380.6158859228593139No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49510.4593629412588827No Hit
GCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAG25500.23659371848316518No Hit
GTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTAGTTGT23540.21840847580759642No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTG23370.21683118435104198No Hit
GGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGC21440.19892428722662986No Hit
GCTGGCTGTAGAGGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGC21300.1976253413212321No Hit
GGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTGCTGGCTG20370.1889966292353755No Hit
TGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGGACCAGAGGTTGTG17500.1623682381747212No Hit
GCTGGTGGACTGGTAGGTGGAAGAATCCTCAGGGTTGAAGCGGTTGTGGT16870.15652298160043127No Hit
CCCTTCTTCTTTCCTTCTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTC16450.15262614388423795No Hit
CTGTCGAAGACGGTAAAGTACTGGCGGATGAAGACATCACCCAGGATCCA15980.14826539691611687No Hit
CCGCTCTGTTTTCCCTGAACCAGGAGTCTCCAGATGTCAACACAGAAGTA15770.14631697805802021No Hit
GTCACCTTCATCTTACGGATTTTAGCTCGAACTTTGGTTTCCACCTGTCT15710.1457602869557069No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA14680.13620375636599472No Hit
GGCAGAGGTTGTGCTGGTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAA13440.12469880691818588No Hit
GGGGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACG13300.12339986101278812No Hit
GTCAACAATGGCCTGGCAGCCCTCAGCGCAGGCGATGGCCTCTCCGTTCA12830.11903911404466704No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC12440.11542062187963038No Hit
CTCCCGCGTCAAGAGAAAAGCCAACATGTTTTTCCTCCAATGCATAAAAG12350.1145855852261604No Hit
GCCCTTTTTAATTCTGTCCACGTCAAGAGCCAAGCCAAGGTACTGTTCCT12170.1129155119192204No Hit
GTGCTGGTGGTGGGAACAGGGCTGGGAGTACTTCCAGGACCAGAGGTTGT11790.10938980160456932No Hit
GGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTAGTTCCAGG11570.10734860089608711No Hit
GCCTGTCATGCTGCCGGTGCCGTAGGTGATGGAGACTGTCTCGCTGGTGG11340.10521461833721935No Hit
GGCCGAGATTGTGCTGGTTGTAGGGGCAGAGGTTGTGCTTGTTGTGGGAG11280.10465792723490601No Hit
GTTGTAGGAGCAGAGGTTGTGCTGGTGGTGGGAACAGGGCTGGGAGTACT11230.10419401798297824No Hit
CTCCGATGTCGCTCTGGATGTTGGCAATGGGGCTGGTTGGGCCGGTCAGC10880.10094665321948382No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)