FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077797
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA44980.4173327630342263No Hit
CGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33890.3144376909566458No Hit
CGTTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC29380.27259307643276054No Hit
CGGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCT26810.2487481408836729No Hit
CGCAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCA21760.20189330643896763No Hit
CGGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAG17620.16348162037934788No Hit
CGGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA17600.16329605667857675No Hit
CGACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTACAACC17250.16004869191508234No Hit
CGCCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGC16500.15309005313616572No Hit
CGTGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAG16240.1506777250261413No Hit
CGTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACG15710.1457602869557069No Hit
CGGACAGTGGACTGTTATGGGTGTAGGTGAATTGGCTTATTTTGTCTGTC15470.14353352254645355No Hit
CGCAATACTCTGGCTGACCATCGTCATCGTGGGACTGACTTTGGTGGAAG15400.14288404959375467No Hit
CGCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTG14890.13815217522409134No Hit
CGCAACCACAACACTCTCACCTGCCAGCATGACAAGCCTAGGCGTCGGTG14600.13546150156291026No Hit
CGATCTGCTCCTACAAGCAGCATGACCTCTGGTCCTGGAACTACTCCCAG14280.1324924823505725No Hit
CGAGCACAACCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACC13510.1253482798708848No Hit
CGCTTCTCCCTCGAGTTGGGACCCGGGAAGAACCATGAAGTGGCTGCTGC12890.11959580514698037No Hit
CGCTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCT12570.11662678593464262No Hit
CGGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGT12290.11402889412384708No Hit
CGCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTC12280.11393611227346152No Hit
CGAGGCTCAGCAGGGAGCTGCTGGATGAGAAAGAGCCTGAAGTCTTGCAG12200.11319385747037708No Hit
CGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG11880.1102248382580393No Hit
CGCTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAAC11740.10892589235264155No Hit
CGCACCACCAGCACAACCTCTGCTCCTATAACCAGCACAACCTCTGGTCC11510.10679190979377379No Hit
CGCCTGCCCAGGATTTCACCGTCGTCTTTGACACCGGCTCCTCCAACCTG11120.10317341762873714No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11080.10280229022719492No Hit
CGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA11070.10270950837680937No Hit
CGTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCC10960.10168890802256826No Hit
CGCACCACCAGCACAACCTCTGCTCCTACAACTAGCACAACCTCTGGTCC10910.10122499877064048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)