FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26269502
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC5331922.029699687493124Illumina Single End PCR Primer 1 (97% over 49bp)
ATCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTA543390.20685203701234992No Hit
ATCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTT310950.11836920243101677No Hit
ATCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG301320.1147033544830808No Hit
ATCCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGC283500.10791982276633946No Hit
ATCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAG271190.1032337803739104No Hit
ATCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATT264010.10050057286963415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)