FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006609709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006609709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28559712
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC929390.3254199482123629Illumina Single End PCR Primer 1 (97% over 49bp)
ATCCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCC549650.19245642252975098No Hit
ATCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATT540530.1892631130173862No Hit
ATCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC424370.14859043396516042No Hit
ATCCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT415020.14531659142781272No Hit
ATCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGG370690.12979472622132884No Hit
ATCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACC358880.12565953046025113No Hit
ATCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC344460.12061045993741112No Hit
ATCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGG327990.1148435950614628No Hit
ATCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCG307910.10781271183687006No Hit
ATCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA305640.10701788589464768No Hit
ATCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGC298450.10450035350496531No Hit
ATCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGC297070.104017155355068No Hit
ATCTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCC291490.10206335414026584No Hit
ATCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTG287310.10059975394709864No Hit
ATCAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT285750.10005352995156253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)