FastQCFastQC Report
Tue 9 Aug 2022
EGAF00006620197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006620197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54778069
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3130160.5714257653003432No Hit
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC1949440.3558796495728975No Hit
CTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTG1750140.31949647586153507No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA1675800.30592535125690534No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG1491580.2722951040862722No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1431350.26129982785629047No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC1416080.25851221590158646No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC1148280.20962403767829058No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG1115640.2036654486670569No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC1103660.20147844203854648No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC1061150.19371803704873206No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA965930.17633516800309262No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG949790.17338873336334656No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC921950.1683064074420002No Hit
GCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGT869320.15869854777100667No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC858350.1566959215009934No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG833660.1521886432323856No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC799420.14593796652452282No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC777020.14184873877171536TruSeq Adapter, Index 6 (100% over 50bp)
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT765320.13971284749011506No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA760390.13881285227487666No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT677440.12366993075276166No Hit
CGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTC669750.12226608426083804No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC641100.11703588894307318No Hit
TGCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGG590620.10782052211442503No Hit
CAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGC569290.10392662800873832No Hit
CCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATT564590.10306862039989033No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG554140.10116092263128151No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC552910.10093638021449788No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)