FastQCFastQC Report
Tue 9 Aug 2022
EGAF00006620199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006620199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50954573
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC2688450.5276170207529753No Hit
CTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTG2302390.4518514952524477No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2037630.3998914876590174No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC1646780.3231859091430322No Hit
GCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGT1265400.24833884880165713No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG1094350.2147697322475845No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA956890.18779276199606265No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC936680.18382648403314064No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT918590.18027626293718524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC866270.17000829346563262TruSeq Adapter, Index 6 (100% over 50bp)
TGCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGG846190.1660675284237982No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC823210.16155762898847176No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG801890.1573735099301097No Hit
ATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTG776990.15248680427564373No Hit
AGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCC764220.14998065041188746No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC705000.1383585335903021No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT702990.1379640645796404No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC693750.13615068465003133No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC693020.13600741978546263No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA664970.13050251642772082No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG654530.12845363261114953No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGG638180.12524489215128937No Hit
CGCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGG613760.12045238805160824No Hit
CCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG604980.11872928461200136No Hit
CAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGA599930.1177382057543687No Hit
CTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGGGCTGCCTG591970.11617602997085266No Hit
AAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCC577350.11330680761469634No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG553080.1085437415008855No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGG546400.1072327698634625No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC537020.10539191448037451No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC511480.100379606752862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)