FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370
Sequences flagged as poor quality0
Sequence length506-3940
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC7675.736724008975318No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA7635.706806282722513No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC4623.4554973821989527No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC2772.0718025430067315No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC2461.8399401645474944No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1561.1667913238593868No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1501.1219147344801794No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1411.0545998504113687No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1300.9723261032161555No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1290.9648466716529543No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1260.9424083769633508No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1200.8975317875841435No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT1150.8601346297681376No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA1110.8302169035153328No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1000.7479431563201197No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC980.7329842931937173No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG970.7255048616305161No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC940.7030665669409125No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG940.7030665669409125No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG880.6581899775617053No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG850.6357516828721017No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG840.6282722513089005No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA830.6207928197456993No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG800.5983545250560958No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG790.5908750934928946No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACTATTCACACCTTCATTACC790.5908750934928946No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG790.5908750934928946No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG770.5759162303664921No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG750.5609573672400897No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG740.5534779356768885No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA740.5534779356768885No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC720.5385190725504861No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG690.5160807778608826No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG680.5086013462976814No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC670.5011219147344802No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG660.49364248317127896No Hit
TGGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG590.4412864622288706No Hit
GGCTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA590.4412864622288706No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG570.4263275991024682No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG570.4263275991024682No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC560.41884816753926707No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC540.4038893044128646No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC520.38893044128646226No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG510.381451009723261No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAACA500.3739715781600598No Hit
TGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG500.3739715781600598No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG500.3739715781600598No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG490.36649214659685864No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG480.35901271503365745No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG480.35901271503365745No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG470.35153328347045626No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC470.35153328347045626No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG470.35153328347045626No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG460.34405385190725507No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCGGTATTACCCTAGTGCGC430.32161555721765145No Hit
GGCTACCTTGTTACGACTTGACCCCAATCACTAGTCCTACCTTCGACGGC430.32161555721765145No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGTCGC410.30665669409124907No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG400.2991772625280479No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG390.2916978309648467No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC390.2916978309648467No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG380.2842183994016455No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTACCCTAGGCCGA380.2842183994016455No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA370.27673896783844426No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG360.26925953627524307No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG350.2617801047120419No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC350.2617801047120419No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG350.2617801047120419No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT340.2543006731488407No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGCCCCCACCTTCGACGGC340.2543006731488407No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC330.24682124158563948No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG320.23934181002243832No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG320.23934181002243832No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT310.23186237845923707No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC300.22438294689603588No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG300.22438294689603588No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC300.22438294689603588No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG290.21690351533283472No Hit
TGGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGG290.21690351533283472No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG290.21690351533283472No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG290.21690351533283472No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC280.20942408376963353No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTTGTTTTGCCTTGGGCAGC280.20942408376963353No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACGGT280.20942408376963353No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG280.20942408376963353No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTCGCTCTAGGCCGA280.20942408376963353No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGC280.20942408376963353No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG270.2019446522064323No Hit
ATGGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCC270.2019446522064323No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG270.2019446522064323No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC270.2019446522064323No Hit
TGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG260.19446522064323113No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG260.19446522064323113No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC260.19446522064323113No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG260.19446522064323113No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG260.19446522064323113No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG260.19446522064323113No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC240.17950635751682872No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG240.17950635751682872No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG230.17202692595362754No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT230.17202692595362754No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG230.17202692595362754No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG230.17202692595362754No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG220.16454749439042632No Hit
GGCTACCTTGTTACGACTTCACTCCAATCATCAGCTCCGCCTTCGACGGC220.16454749439042632No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC220.16454749439042632No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT220.16454749439042632No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG210.15706806282722513No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG210.15706806282722513No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG200.14958863126402394No Hit
AGCTACCTTGTTACGACTTCACCCCAATCACTATTCACACCTTCATTACC200.14958863126402394No Hit
AGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA190.14210919970082275No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG190.14210919970082275No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC190.14210919970082275No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG190.14210919970082275No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG190.14210919970082275No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG180.13462976813762154No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG180.13462976813762154No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG180.13462976813762154No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG180.13462976813762154No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG180.13462976813762154No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG180.13462976813762154No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA180.13462976813762154No Hit
TAGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG180.13462976813762154No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG170.12715033657442035No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG170.12715033657442035No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACCAT170.12715033657442035No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG160.11967090501121916No Hit
TGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAA160.11967090501121916No Hit
TAGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG160.11967090501121916No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG160.11967090501121916No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG160.11967090501121916No Hit
AGGGTTCGATTCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACAT160.11967090501121916No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG160.11967090501121916No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG160.11967090501121916No Hit
AGGGTTCGATCCTGGCTCAGGATAAACGCTGACAGAATGCTTAACACATG150.11219147344801794No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG150.11219147344801794No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG150.11219147344801794No Hit
ATGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCG150.11219147344801794No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG140.10471204188481677No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA140.10471204188481677No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG140.10471204188481677No Hit
GGTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGAT140.10471204188481677No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG140.10471204188481677No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG140.10471204188481677No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG140.10471204188481677No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG140.10471204188481677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)