FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056
Sequences flagged as poor quality0
Sequence length503-5160
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC5237.412131519274376No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA4105.8106575963718825No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC2874.067460317460317No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC2353.330498866213152No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1782.522675736961451No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1211.7148526077097506No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG971.374716553287982No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC771.0912698412698412No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC711.006235827664399No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG680.9637188208616779No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA670.949546485260771No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC670.949546485260771No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG610.8645124716553287No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG590.8361678004535147No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG560.7936507936507936No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG540.7653061224489796No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG480.6802721088435374No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG440.6235827664399093No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG420.5952380952380952No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA410.5810657596371882No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC400.5668934240362812No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG390.5527210884353742No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG380.5385487528344671No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG370.5243764172335601No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG370.5243764172335601No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG370.5243764172335601No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG360.5102040816326531No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG340.48185941043083896No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG320.45351473922902497No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG310.43934240362811794No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG310.43934240362811794No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG300.4251700680272109No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG300.4251700680272109No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG280.3968253968253968No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG280.3968253968253968No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG270.3826530612244898No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG270.3826530612244898No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG270.3826530612244898No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG260.36848072562358275No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG260.36848072562358275No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG250.3543083900226758No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG240.3401360544217687No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC240.3401360544217687No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG230.32596371882086167No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.31179138321995464No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG220.31179138321995464No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG220.31179138321995464No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG220.31179138321995464No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG210.2976190476190476No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG210.2976190476190476No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG210.2976190476190476No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG210.2976190476190476No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC200.2834467120181406No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG200.2834467120181406No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG190.26927437641723356No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATC190.26927437641723356No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG180.25510204081632654No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG170.24092970521541948No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG160.22675736961451248No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA160.22675736961451248No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG160.22675736961451248No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG160.22675736961451248No Hit
GGTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGAT150.21258503401360546No Hit
AGAGTTCGATTCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACAT150.21258503401360546No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG150.21258503401360546No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG140.1984126984126984No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA140.1984126984126984No Hit
GGCTACCTTGTTACGACTTGGTGAGCCCGCGGCATGGTGAGCCCAAGACA140.1984126984126984No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG140.1984126984126984No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC140.1984126984126984No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTGCCGTGGTAATC130.18424036281179137No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC130.18424036281179137No Hit
GGCTACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT130.18424036281179137No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG130.18424036281179137No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG130.18424036281179137No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC130.18424036281179137No Hit
TGGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG120.17006802721088435No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG120.17006802721088435No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG120.17006802721088435No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAACG120.17006802721088435No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG110.15589569160997732No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG110.15589569160997732No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT110.15589569160997732No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG100.1417233560090703No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGGTTTTGCCCTAGGCCG100.1417233560090703No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA100.1417233560090703No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG90.12755102040816327No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG90.12755102040816327No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTCTTGCCCTAGGTCGC90.12755102040816327No Hit
AGGGTTCGATTCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACAT90.12755102040816327No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC90.12755102040816327No Hit
AGGGTTTGATCATGGCTCAGACAGCCGACCCCGACAAGCCCTGAGCGCCC80.11337868480725624No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGCT80.11337868480725624No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG80.11337868480725624No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCTACAGGCTTAACACATG80.11337868480725624No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTAGCCGTGGTAAT80.11337868480725624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)