FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12849
Sequences flagged as poor quality0
Sequence length525-3887
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC7886.132772978441902No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC7826.086076737489298No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC5264.093703790178224No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC4713.6656549147793602No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC2832.20250603159779No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC2672.0779827223908476No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2451.906763172231302No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1951.5176278309596078No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1451.1284924896879136No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1361.0584481282590086No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1361.0584481282590086No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1250.9728383531792357No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1240.9650556463538018No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1130.8794458712740292No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1120.8716631644485952No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1100.8560977507977274No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1100.8560977507977274No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1080.8405323371468596No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG1070.8327496303214258No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG970.754922562067087No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG940.7315744415907852No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG800.622616546034711No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG790.614833839209277No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG710.5525721846058058No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG710.5525721846058058No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG660.5136586504786365No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG660.5136586504786365No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA630.49031053000233477No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG610.47474511635146704No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG600.4669624095260332No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC580.4513969958751654No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT570.44361428904973155No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG550.4280488753988637No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC540.4202661685734298No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG490.3813526344462604No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGTCGC470.3657872207953926No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTCGCTCTAGGCCGA460.35800451396995875No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG440.34243910031909097No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG440.34243910031909097No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTCGCTCTAGGCCGA440.34243910031909097No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG430.33465639349365706No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG420.3268736866682232No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA420.3268736866682232No Hit
AGGGTTCGATTCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT410.31909097984278934No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG390.30352556619192156No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG390.30352556619192156No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG380.29574285936648764No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG380.29574285936648764No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA380.29574285936648764No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG370.2879601525410538No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG370.2879601525410538No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG370.2879601525410538No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA360.2801774457156199No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG360.2801774457156199No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGTCGC360.2801774457156199No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG350.272394738890186No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG350.272394738890186No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG320.24904661841388437No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC320.24904661841388437No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG310.24126391158845045No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG310.24126391158845045No Hit
AGGGTTCGATTCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT300.2334812047630166No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG290.2256984979375827No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA290.2256984979375827No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG280.2179157911121488No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG280.2179157911121488No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC270.2101330842867149No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG270.2101330842867149No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG270.2101330842867149No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG250.19456767063584715No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC250.19456767063584715No Hit
GGTTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC250.19456767063584715No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC240.18678496381041326No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG240.18678496381041326No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA240.18678496381041326No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG230.17900225698497937No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG230.17900225698497937No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGTCGC230.17900225698497937No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC230.17900225698497937No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.17121955015954549No Hit
TGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG210.1634368433341116No Hit
AGGGTTTGATTCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT210.1634368433341116No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTCGCTCTAGGCCGA210.1634368433341116No Hit
GTGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAA210.1634368433341116No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC200.15565413650867774No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG200.15565413650867774No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTCGCTCTAGGCCGA200.15565413650867774No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG190.14787142968324382No Hit
TGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG190.14787142968324382No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC190.14787142968324382No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG190.14787142968324382No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATTTGTTTTGCCTTGGGCAGC180.14008872285780996No Hit
GGTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGAT180.14008872285780996No Hit
AGGGTTTGATTCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT180.14008872285780996No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG180.14008872285780996No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA180.14008872285780996No Hit
AGGGTTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT180.14008872285780996No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG180.14008872285780996No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA170.13230601603237607No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA170.13230601603237607No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG170.13230601603237607No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG170.13230601603237607No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG170.13230601603237607No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC160.12452330920694218No Hit
AGAGTTCGATTCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT160.12452330920694218No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG160.12452330920694218No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC160.12452330920694218No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTTGTTTTGCCTTGGGCAGC160.12452330920694218No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG160.12452330920694218No Hit
TGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG150.1167406023815083No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG150.1167406023815083No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC150.1167406023815083No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG150.1167406023815083No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG150.1167406023815083No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT140.1089578955560744No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG140.1089578955560744No Hit
AGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG140.1089578955560744No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG140.1089578955560744No Hit
AGCTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC140.1089578955560744No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG140.1089578955560744No Hit
AGAGTTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT140.1089578955560744No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG140.1089578955560744No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG140.1089578955560744No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTAACCCTACCTTCGACGCC140.1089578955560744No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT140.1089578955560744No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGTCCG140.1089578955560744No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC130.10117518873064052No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC130.10117518873064052No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG130.10117518873064052No Hit
AGGGTTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT130.10117518873064052No Hit
GTGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAA130.10117518873064052No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC130.10117518873064052No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG130.10117518873064052No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG130.10117518873064052No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA130.10117518873064052No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG130.10117518873064052No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG130.10117518873064052No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG130.10117518873064052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)