FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5756
Sequences flagged as poor quality0
Sequence length508-3492
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC5439.43363446838082No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC3706.428075052119528No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC3085.350938151494093No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC2063.5788742182070883No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1432.4843641417651146No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1332.3106323835997222No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1111.928422515635858No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1111.928422515635858No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC681.18137595552467No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG581.0076441973592773No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG560.9728978457261988No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG540.9381514940931202No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA460.7991660875608061No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA450.7817929117442668No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG450.7817929117442668No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG440.7644197359277276No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG410.7123002084781098No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG410.7123002084781098No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC390.6775538568450313No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA370.6428075052119527No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC360.6254343293954135No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC340.590687977762335No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT330.5733148019457956No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG310.5385684503127172No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG310.5385684503127172No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG300.5211952744961779No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG290.5038220986796387No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG290.5038220986796387No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG290.5038220986796387No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC280.4864489228630994No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG280.4864489228630994No Hit
AGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC270.4690757470465601No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA270.4690757470465601No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC270.4690757470465601No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG260.45170257123002083No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA260.45170257123002083No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC250.4343293954134816No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG250.4343293954134816No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG240.4169562195969423No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG240.4169562195969423No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA240.4169562195969423No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC230.39958304378040305No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG220.3822098679638638No Hit
GGTTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC220.3822098679638638No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC200.34746351633078526No Hit
AGAGTTCGATCCTGGCTCAGGACTGAACGCTGGCGGCGTGCCTAATACAT200.34746351633078526No Hit
AGTTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC190.330090340514246No Hit
GGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG190.330090340514246No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG190.330090340514246No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG180.3127171646977068No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG180.3127171646977068No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT170.2953439888811675No Hit
AGGGTTCGATCCTGGCTCAGGACTGAACGCTGGCGGCGTGCCTAATACAT170.2953439888811675No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG170.2953439888811675No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG170.2953439888811675No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA170.2953439888811675No Hit
AGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT170.2953439888811675No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG160.27797081306462823No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT160.27797081306462823No Hit
GAGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG150.26059763724808893No Hit
AGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGC140.2432244614315497No Hit
AGGGTTCGATCATGGCTCAGGACTGAACGCTGGCGGCGTGCCTAATACAT140.2432244614315497No Hit
GGCTACCTTGTTACGACTTCACCCCCCTTACCAACCACACCTTCGGCGTC130.22585128561501042No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG130.22585128561501042No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG130.22585128561501042No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG130.22585128561501042No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG120.20847810979847115No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA120.20847810979847115No Hit
AGAGTTCGATTCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT120.20847810979847115No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG110.1911049339819319No Hit
AGAGTTCGATCATGGCTCAGGACTGAACGCTGGCGGCGTGCCTAATACAT110.1911049339819319No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG110.1911049339819319No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC110.1911049339819319No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG110.1911049339819319No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG100.17373175816539263No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT100.17373175816539263No Hit
AGGGTTCGATTCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT100.17373175816539263No Hit
AGAGTTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT100.17373175816539263No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG100.17373175816539263No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG100.17373175816539263No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT100.17373175816539263No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG100.17373175816539263No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG90.1563585823488534No Hit
GGTTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA90.1563585823488534No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG90.1563585823488534No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT90.1563585823488534No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG90.1563585823488534No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG90.1563585823488534No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG90.1563585823488534No Hit
GGCTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA80.13898540653231412No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG80.13898540653231412No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG80.13898540653231412No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT80.13898540653231412No Hit
AGGGTTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT80.13898540653231412No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG80.13898540653231412No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG80.13898540653231412No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG80.13898540653231412No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG80.13898540653231412No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG80.13898540653231412No Hit
GGGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG70.12161223071577484No Hit
GGCTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC70.12161223071577484No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCTACAGGCTTAACACATG70.12161223071577484No Hit
GGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT70.12161223071577484No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT70.12161223071577484No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG70.12161223071577484No Hit
GGTTACCTTGTTACGACTTCACCCCCCTTACCAACCACACCTTCGGCGTC70.12161223071577484No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC70.12161223071577484No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG70.12161223071577484No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG70.12161223071577484No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG70.12161223071577484No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC60.10423905489923557No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT60.10423905489923557No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGT60.10423905489923557No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG60.10423905489923557No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG60.10423905489923557No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG60.10423905489923557No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG60.10423905489923557No Hit
AGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT60.10423905489923557No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG60.10423905489923557No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG60.10423905489923557No Hit
AGGGTTCGATTCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT60.10423905489923557No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG60.10423905489923557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)