FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8465
Sequences flagged as poor quality0
Sequence length509-4822
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATC2162.5516834022445365No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1511.7838157117542823No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1511.7838157117542823No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1461.724748966331955No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATC1181.3939751919669225No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATC1021.2049616066154754No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1011.19314825753101No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG981.1577082102776137No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA921.086828115770821No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATC901.06320141760189No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC901.06320141760189No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG891.0513880685174246No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC800.9450679267572357No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC790.9332545776727702No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA790.9332545776727702No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC770.9096278795038394No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG750.8860011813349086No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC690.8151210868281158No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG690.8151210868281158No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA660.7796810395747195No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG610.7206142941523922No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG600.7088009450679268No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG590.6969875959834613No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC550.6497341996455995No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG550.6497341996455995No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG500.5906674542232723No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC490.5788541051388069No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC490.5788541051388069No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG460.5434140578854105No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG450.531600708800945No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC420.4961606615475487No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAACCCTGCCGTGGTAATC410.48434731246308327No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG400.47253396337861786No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTGCCGTGGTAATC370.43709391612522147No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG360.425280567040756No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG340.4016538688718252No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC330.38984051978735973No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC330.38984051978735973No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTTAGC330.38984051978735973No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC300.3544004725339634No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG300.3544004725339634No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG290.34258712344949793No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC290.34258712344949793No Hit
AGCTACCTTGTTACGACTTAGACACACACGCACATGGCTACCTTGTTACG280.33077377436503247No Hit
AGGGTTCGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG270.31896042528056706No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC270.31896042528056706No Hit
AGAGTTCGATCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG270.31896042528056706No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC270.31896042528056706No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG260.3071470761961016No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG250.29533372711163614No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG240.2835203780271707No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG240.2835203780271707No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG240.2835203780271707No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA230.27170702894270526No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG230.27170702894270526No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG220.2598936798582398No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG220.2598936798582398No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG210.24808033077377434No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAACCCTACCGTGGTAATCG210.24808033077377434No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG200.23626698168930893No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAACCCTGCCGTGGTAATC200.23626698168930893No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG200.23626698168930893No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC190.2244536326048435No Hit
GGTTACCTTGTTACGACTTAGACACACACGCACATGGTTACCTTGTTACG190.2244536326048435No Hit
GGTTACCTTGTTACGACTTAGACACACACGCACATGGCTACCTTGTTACG190.2244536326048435No Hit
AGAGTTCGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG190.2244536326048435No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG190.2244536326048435No Hit
AGTTACCTTGTTACGACTTAGACACACACGCACATGGTTACCTTGTTACG190.2244536326048435No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG190.2244536326048435No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA190.2244536326048435No Hit
AGCTACCTTGTTACGACTTAGACACACACGCACATGGTTACCTTGTTACG180.212640283520378No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC180.212640283520378No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC180.212640283520378No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG180.212640283520378No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACGGT180.212640283520378No Hit
GGTTACCTTGTTACGACTTCACCCTCCTTACCAAACGTACCTTCGGCACC180.212640283520378No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG180.212640283520378No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC170.2008269344359126No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG170.2008269344359126No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG170.2008269344359126No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG170.2008269344359126No Hit
GGCTACCTTGTTACGACTTAGACACACACGCACATGGTTACCTTGTTACG170.2008269344359126No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG170.2008269344359126No Hit
AGGGTTCGATCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG170.2008269344359126No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG160.18901358535144713No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG160.18901358535144713No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC160.18901358535144713No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG160.18901358535144713No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTGCCGTGGTAATC160.18901358535144713No Hit
AGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC160.18901358535144713No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC150.1772002362669817No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC150.1772002362669817No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG150.1772002362669817No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT150.1772002362669817No Hit
GGCTACCTTGTTACGACTTAGACACACACGCACATGGCTACCTTGTTACG150.1772002362669817No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG140.16538688718251623No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG140.16538688718251623No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG140.16538688718251623No Hit
GGCTACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT140.16538688718251623No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG140.16538688718251623No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG140.16538688718251623No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG140.16538688718251623No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG130.1535735380980508No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA130.1535735380980508No Hit
AGTTACCTTGTTACGACTTAGACACACACGCACATGGCTACCTTGTTACG130.1535735380980508No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG130.1535735380980508No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG130.1535735380980508No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG120.14176018901358536No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG120.14176018901358536No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC120.14176018901358536No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG120.14176018901358536No Hit
GGCTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA120.14176018901358536No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC120.14176018901358536No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC110.1299468399291199No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAACCCTAGCCGTGGTAAT110.1299468399291199No Hit
AGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC110.1299468399291199No Hit
AGCTACCTTGTTACGACTTAGACACACACGCACATAGGGTTCGATCCTGG110.1299468399291199No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC100.11813349084465447No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG100.11813349084465447No Hit
AGAGTTTGATCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG100.11813349084465447No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG100.11813349084465447No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA100.11813349084465447No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA100.11813349084465447No Hit
GGTTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC100.11813349084465447No Hit
AGCTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA100.11813349084465447No Hit
GGTTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA90.106320141760189No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG90.106320141760189No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTGCCCTAGGCCGA90.106320141760189No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA90.106320141760189No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT90.106320141760189No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG90.106320141760189No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG90.106320141760189No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG90.106320141760189No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGCT90.106320141760189No Hit
GGTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGAT90.106320141760189No Hit
AGCTACCTTGTTACGACTTAGACACACACGCACATAGCTACCTTGTTACG90.106320141760189No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG90.106320141760189No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG90.106320141760189No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCTACAGGCTTAACACATG90.106320141760189No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG90.106320141760189No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCGGTCTCGCCCTAGGCCGA90.106320141760189No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG90.106320141760189No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAACCCTAGCCGTGGTAAT90.106320141760189No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC90.106320141760189No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG90.106320141760189No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG90.106320141760189No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC90.106320141760189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)