FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15163
Sequences flagged as poor quality0
Sequence length503-4882
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC164910.875156631273494No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC8435.559585833937875No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC6514.293345644001847No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG3252.1433753215062983No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC3062.0180703027105453No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2181.4377102156565325No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2161.424520213678032No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1931.2728351909252786No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1871.2332651849897778No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1541.0156301523445228No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1531.0090351513552727No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC1470.9694651454197718No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1450.9562751434412715No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1440.9496801424520214No Hit
GGCTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC1340.8837301325595199No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1240.8177801226670185No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1210.797995119699268No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1200.7914001187100178No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG1180.7782101167315175No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT1170.7716151157422674No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1160.7650201147530172No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1150.7584251137637671No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1110.7320451098067665No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA1050.6924751038712657No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1030.6792851018927653No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC1030.6792851018927653No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC980.6463100969465145No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG920.6067400910110137No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAGCCCTACCGTGGTAAGC910.6001450900217635No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG890.5869550880432632No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG890.5869550880432632No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG880.580360087054013No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG860.5671700850755128No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC840.5539800830970124No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC830.5473850821077623No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG790.5210050781507617No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC750.4946250741937611No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG740.48803007320451103No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG690.4550550682582602No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG690.4550550682582602No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG680.44846006726901005No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC670.44186506627975997No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA600.3957000593550089No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG600.3957000593550089No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC600.3957000593550089No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG590.38910505836575876No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC580.3825100573765086No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG580.3825100573765086No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG570.3759150563872585No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG560.3693200553980083No Hit
AGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG560.3693200553980083No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT550.3627250544087582No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG550.3627250544087582No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG550.3627250544087582No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC520.3429400514410077No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG510.33634505045175755No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG510.33634505045175755No Hit
AGCTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC500.3297500494625074No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG480.3165600474840071No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACGGT470.309965046494757No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG460.30337004550550684No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG450.2967750445162567No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC450.2967750445162567No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC430.2835850425377564No Hit
AGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT430.2835850425377564No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG430.2835850425377564No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG430.2835850425377564No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC410.2703950405592561No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG390.2572050385807558No Hit
AGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA390.2572050385807558No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG380.25061003759150563No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG380.25061003759150563No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG380.25061003759150563No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG370.24401503660225551No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG370.24401503660225551No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG370.24401503660225551No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG360.23742003561300534No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG350.2308250346237552No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA340.22423003363450503No Hit
GGCTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC330.2176350326452549No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG330.2176350326452549No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG320.21104003165600474No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG320.21104003165600474No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG310.20444503066675462No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG310.20444503066675462No Hit
AGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC300.19785002967750445No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG290.1912550286882543No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAGCCCTACCGTGGTAAGC290.1912550286882543No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC290.1912550286882543No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA280.18466002769900414No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG270.178065026709754No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG270.178065026709754No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTCACCCTAGGCCGA260.17147002572050385No Hit
GGCTACCTTGTTACGACTTAGTCCCAATCGCCAGTCCCACCTTCGACGGC260.17147002572050385No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC250.1648750247312537No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA250.1648750247312537No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG250.1648750247312537No Hit
AGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG250.1648750247312537No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG250.1648750247312537No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG250.1648750247312537No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG240.15828002374200356No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG240.15828002374200356No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG230.15168502275275342No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG230.15168502275275342No Hit
AGCTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC230.15168502275275342No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG230.15168502275275342No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG230.15168502275275342No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG230.15168502275275342No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG230.15168502275275342No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.14509002176350325No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC220.14509002176350325No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG220.14509002176350325No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG220.14509002176350325No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT220.14509002176350325No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG220.14509002176350325No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT210.1384950207742531No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG210.1384950207742531No Hit
GGCTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA210.1384950207742531No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA200.13190001978500296No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG200.13190001978500296No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG200.13190001978500296No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG200.13190001978500296No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG190.12530501879575281No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG190.12530501879575281No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG190.12530501879575281No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG180.11871001780650267No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCAGTTTTACCCTAGGCCGA180.11871001780650267No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG180.11871001780650267No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG180.11871001780650267No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG170.11211501681725251No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCATCGGTTTTACCCTAGGTCGC170.11211501681725251No Hit
AGGGTTCGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATG170.11211501681725251No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG170.11211501681725251No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTCGACGGC170.11211501681725251No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG170.11211501681725251No Hit
AAGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG170.11211501681725251No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC160.10552001582800237No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG160.10552001582800237No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG160.10552001582800237No Hit
AGGGTTCGATTCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT160.10552001582800237No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC160.10552001582800237No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC160.10552001582800237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)