FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10270
Sequences flagged as poor quality0
Sequence length503-4609
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC5425.277507302823759No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC4844.712755598831548No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC4614.488802336903603No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC4594.469328140214216No Hit
AGAGTTTGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG3303.213242453748783No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG3253.1645569620253164No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA2532.4634858812074No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA2182.122687439143135No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1941.8889970788704964No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1741.6942551119766307No Hit
AGAGTTTGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1651.6066212268743916No Hit
AGGGTTTGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1471.4313534566699124No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC1251.2171372930866602No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1231.1976630963972736No Hit
AGGGTTTGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1131.100292112950341No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC1111.0808179162609541No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1051.0223953261927945No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1041.0126582278481013No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC940.9152872444011685No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC840.8179162609542356No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG700.6815968841285297No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG630.6134371957156768No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA570.555014605647517No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG570.555014605647517No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA560.5452775073028238No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC530.5160662122687439No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA510.4965920155793574No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC500.4868549172346641No Hit
AGTTACCTTGTTACGACTTAGCGCTGCGACACGCGGGTTACCTTGTTACG450.4381694255111977No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC440.4284323271665044No Hit
AGAGTTTGATCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG430.4186952288218111No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG420.4089581304771178No Hit
AGAGTTCGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG390.37974683544303794No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA380.37000973709834467No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG370.36027263875365145No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG370.36027263875365145No Hit
AGAGTTTGATTATGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGG360.3505355404089581No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA360.3505355404089581No Hit
AGGGTTTGATCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG330.32132424537487825No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG320.31158714703018503No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA320.31158714703018503No Hit
GAGAGTTTGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACAT310.30185004868549176No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC300.2921129503407984No Hit
AGTTACCTTGTTACGACTTAGCGCTGCGACACGCGAGTTACCTTGTTACG300.2921129503407984No Hit
GAGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT290.28237585199610515No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA260.25316455696202533No Hit
GGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC260.25316455696202533No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG250.24342745861733206No Hit
GAGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG250.24342745861733206No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG250.24342745861733206No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG250.24342745861733206No Hit
AGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC240.23369036027263873No Hit
GGTTACCTTGTTACGACTTAGCGCTGCGACACGCGGGTTACCTTGTTACG240.23369036027263873No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG240.23369036027263873No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG240.23369036027263873No Hit
GAGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGG240.23369036027263873No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC230.22395326192794549No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG230.22395326192794549No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA230.22395326192794549No Hit
AGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA220.2142161635832522No Hit
GGTTACCTTGTTACGACTTAGCGCTGCGACACGCGAGTTACCTTGTTACG220.2142161635832522No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC210.2044790652385589No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC210.2044790652385589No Hit
GGTTACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT210.2044790652385589No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG210.2044790652385589No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG200.19474196689386564No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG200.19474196689386564No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG200.19474196689386564No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT200.19474196689386564No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC190.18500486854917234No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG190.18500486854917234No Hit
AGAGTTCGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG190.18500486854917234No Hit
AGAGTTTGATTCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT180.17526777020447906No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG180.17526777020447906No Hit
GGTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGCT180.17526777020447906No Hit
GGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG170.1655306718597858No Hit
GGTTACCTTGTTACGACTTAGTCCCAATCGCCAGTCCCACCTTCGACCAC170.1655306718597858No Hit
AGTTACCTTGTTACGACTTAGCGCTGCGACACGCGGGCTACCTTGTTACG170.1655306718597858No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG160.15579357351509251No Hit
GGGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGG160.15579357351509251No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG150.1460564751703992No Hit
GAGAGTTTGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACAT150.1460564751703992No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGCC150.1460564751703992No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG150.1460564751703992No Hit
GAGAGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACAT150.1460564751703992No Hit
GAGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT140.13631937682570594No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG140.13631937682570594No Hit
AGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG140.13631937682570594No Hit
AGAGTTTGATTCATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACAT140.13631937682570594No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT140.13631937682570594No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA140.13631937682570594No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA130.12658227848101267No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC130.12658227848101267No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG130.12658227848101267No Hit
GGGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG130.12658227848101267No Hit
AGCTACCTTGTTACGACTTAGCGCTGCGACACGCGGGTTACCTTGTTACG130.12658227848101267No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG130.12658227848101267No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG120.11684518013631937No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA120.11684518013631937No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG120.11684518013631937No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG120.11684518013631937No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG120.11684518013631937No Hit
AGTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGCT120.11684518013631937No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG120.11684518013631937No Hit
AGCTACCTTGTTACGACTTAGCGCTGCGACACGCGAGTTACCTTGTTACG110.1071080817916261No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG110.1071080817916261No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG110.1071080817916261No Hit
AGGGTTCGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG110.1071080817916261No Hit
AGAGTTTGATTATGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG110.1071080817916261No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG110.1071080817916261No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG110.1071080817916261No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG110.1071080817916261No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGAC110.1071080817916261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)