FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11335
Sequences flagged as poor quality0
Sequence length509-4393
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC4493.9611821790913107No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA3923.4583149536832822No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC3463.0524922805469785No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC2712.3908248786943096No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC1861.6409351565946184No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1851.6321129245699162No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1811.5968239964711073No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1791.5791795324217026No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC1741.5350683722981915No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1541.3586237318041463No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1421.2527569475077196No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1231.0851345390383766No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC1110.9792677547419496No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1070.9439788266431407No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1020.8998676665196295No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC990.8734009704455228No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG860.7587119541243935No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC850.7498897220996913No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG830.7322452580502867No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG810.7146007940008822No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG800.7057785619761799No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG800.7057785619761799No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG800.7057785619761799No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA740.6528451698279665No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG730.6440229378032643No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC710.6263784737538598No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC710.6263784737538598No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG700.6175562417291575No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG630.5558006175562417No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG620.5469783855315394No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA620.5469783855315394No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG610.5381561535068372No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG610.5381561535068372No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA600.529333921482135No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG590.5205116894574326No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG590.5205116894574326No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC590.5205116894574326No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG570.5028672254080282No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG560.4940449933833259No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC550.4852227613586237No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG540.47640052933392146No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG520.45875606528451696No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC520.45875606528451696No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG520.45875606528451696No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG510.44993383325981473No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG510.44993383325981473No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG490.4322893692104103No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG470.4146449051610057No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG460.4058226731363035No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG420.3705337450374945No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC410.36171151301279225No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG410.36171151301279225No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG410.36171151301279225No Hit
AAGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG390.34406704896338774No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA390.34406704896338774No Hit
GGCTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC380.33524481693868546No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG380.33524481693868546No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG370.32642258491398324No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG370.32642258491398324No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG350.30877812086457873No Hit
AGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG350.30877812086457873No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA340.2999558888398765No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC340.2999558888398765No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG340.2999558888398765No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG340.2999558888398765No Hit
AAGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGG310.2734891927657697No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG310.2734891927657697No Hit
AGGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG310.2734891927657697No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA310.2734891927657697No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG310.2734891927657697No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG310.2734891927657697No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG310.2734891927657697No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG300.2646669607410675No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG300.2646669607410675No Hit
AAGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG290.2558447287163652No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG290.2558447287163652No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG290.2558447287163652No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG290.2558447287163652No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC280.24702249669166296No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG280.24702249669166296No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA270.23820026466696073No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG260.22937803264225848No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG260.22937803264225848No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA260.22937803264225848No Hit
AGGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGG260.22937803264225848No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG260.22937803264225848No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA240.21173356859285397No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG240.21173356859285397No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG230.20291133656815175No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG230.20291133656815175No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG230.20291133656815175No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.1940891045434495No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.1940891045434495No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG220.1940891045434495No Hit
AGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA210.18526687251874724No Hit
AGCTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC210.18526687251874724No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG200.17644464049404499No Hit
GAGGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCC200.17644464049404499No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT200.17644464049404499No Hit
AAGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG200.17644464049404499No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA190.16762240846934273No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGCT190.16762240846934273No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG190.16762240846934273No Hit
AGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG190.16762240846934273No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG190.16762240846934273No Hit
GGCTACCTTGTTACGACTTGACCCCAATCACTAGTCCTACCTTCGACGGC190.16762240846934273No Hit
AGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG190.16762240846934273No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG190.16762240846934273No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC180.1588001764446405No Hit
AGCTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC170.14997794441993825No Hit
AGGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCG170.14997794441993825No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG160.141155712395236No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG160.141155712395236No Hit
GGTTACCTTGTTACGACTTAGTCCCAATCGCCAATCCCACCTTAGACGGC160.141155712395236No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG160.141155712395236No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG150.13233348037053375No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG150.13233348037053375No Hit
GGTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGAT150.13233348037053375No Hit
AGGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAA150.13233348037053375No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG150.13233348037053375No Hit
GGTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGCT140.12351124834583148No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG140.12351124834583148No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG140.12351124834583148No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA140.12351124834583148No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT140.12351124834583148No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA140.12351124834583148No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC140.12351124834583148No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG140.12351124834583148No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG140.12351124834583148No Hit
GAGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAA140.12351124834583148No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG140.12351124834583148No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG140.12351124834583148No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG130.11468901632112924No Hit
GAGGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCG130.11468901632112924No Hit
AAGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACAT130.11468901632112924No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC130.11468901632112924No Hit
AAGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACAT130.11468901632112924No Hit
AAGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG130.11468901632112924No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG130.11468901632112924No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG120.10586678429642699No Hit
AAGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAA120.10586678429642699No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG120.10586678429642699No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA120.10586678429642699No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA120.10586678429642699No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC120.10586678429642699No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG120.10586678429642699No Hit
AAGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACAT120.10586678429642699No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG120.10586678429642699No Hit
AAGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACAT120.10586678429642699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)