FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15086
Sequences flagged as poor quality0
Sequence length512-4310
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC4913.2546732069468383No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC4903.2480445446108974No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA4382.903354103141986No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC2991.9819700384462415No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC2931.9421980644305978No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC2851.8891687657430731No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2711.7963674930399045No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC2621.736709532016439No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2621.736709532016439No Hit
AGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA2421.6041362852976269No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG2231.4781917009147554No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG2101.3920190905475276No Hit
GGTTACCTTGTTACGACTTAGCCCTAGTTACTTGTTTTACCCTAGGCAGC2081.3787617658756464No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2001.3257324671881214No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1891.2528171814927749No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC1751.1600159087896063No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC1751.1600159087896063No Hit
AGAGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1521.0075566750629723No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1521.0075566750629723No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG1280.8484687790003976No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG1250.828582791992576No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC1210.8020681426488134No Hit
AGGGTTTGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1200.7954394803128728No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1200.7954394803128728No Hit
AGGGTTTGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1140.7556675062972292No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1120.742410181625348No Hit
AGAGTTTGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1050.6960095452737638No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1030.6827522206018826No Hit
AGTTACCTTGTTACGACTTAGCCCTAGTTACTTGTTTTACCCTAGGCAGC1020.676123558265942No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCGGAAGGCCTAACACATG940.6230942595784171No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG900.5965796102346547No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGT880.5833222855627734No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG800.5302929868752485No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC780.5170356622033674No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCGGAAGGCCTAACACATG770.5104069998674268No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA750.49714967519554554No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG670.4441203765080207No Hit
AGGGTTTGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG650.43086305183613943No Hit
AGGGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG650.43086305183613943No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTGTCCCACCGTGACCGGC640.4242343895001988No Hit
GGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC610.404348402492377No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC610.404348402492377No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC600.3977197401564364No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC560.371205090812674No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA550.3645764284767334No Hit
AGAGTTTGATTCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG540.35794776614079277No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG520.3446904414689116No Hit
AGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG510.338061779132971No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG510.338061779132971No Hit
AGGGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG490.32480445446108974No Hit
AGAGTTTGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG470.31154712978920857No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC460.3049184674532679No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTTTTACCCTAGGCAGC450.29828980511732733No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA440.2916611427813867No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCGGAAGGCCTAACACATG400.26514649343762425No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGGC400.26514649343762425No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG390.2585178311016837No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCGGAAGGCCTAACACATG390.2585178311016837No Hit
AGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG380.2518891687657431No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG380.2518891687657431No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC370.2452605064298025No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACCAT360.23863184409386187No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC350.23200318175792123No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG350.23200318175792123No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG340.22537451942198067No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG340.22537451942198067No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG330.21874585708604005No Hit
TGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG330.21874585708604005No Hit
AGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGGTACC330.21874585708604005No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA320.2121171947500994No Hit
AGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA320.2121171947500994No Hit
AGGGTTTGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG310.20548853241415885No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGT310.20548853241415885No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG310.20548853241415885No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG310.20548853241415885No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG310.20548853241415885No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG300.1988598700782182No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC300.1988598700782182No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA300.1988598700782182No Hit
TGGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG290.19223120774227762No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC280.185602545406337No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG280.185602545406337No Hit
AGAGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG270.17897388307039638No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCCGCCCTGCCTTCGGCAGC270.17897388307039638No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG270.17897388307039638No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG270.17897388307039638No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG270.17897388307039638No Hit
GGTTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC260.1723452207344558No Hit
AGGGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG260.1723452207344558No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC260.1723452207344558No Hit
TAGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGG250.16571655839851518No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG250.16571655839851518No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAACA250.16571655839851518No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG250.16571655839851518No Hit
TGGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAA240.1590878960625746No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC240.1590878960625746No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG240.1590878960625746No Hit
AGGGTTTGATTATGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATG240.1590878960625746No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG230.15245923372663395No Hit
TGGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGC230.15245923372663395No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTCACCCTAGGCCGA230.15245923372663395No Hit
AGAGTTTGATTATGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG230.15245923372663395No Hit
AGAGTTTGATTATGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATG230.15245923372663395No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG230.15245923372663395No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG230.15245923372663395No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG220.14583057139069336No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACTAGTTTTACCCTAAACAGC220.14583057139069336No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG220.14583057139069336No Hit
GGCTACCTTGTTACGACTTAGCCCTAGTTACTTGTTTTACCCTAGGCAGC220.14583057139069336No Hit
TAGAGTTTGATTATGGCTCAGGATGAACGCTGACAGAATGCTTAACACAT220.14583057139069336No Hit
AGTTACCTTGTTACGACTTCACCCCAGTTATCGACGCCACCTTCGACGAC210.13920190905475277No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTACCCTAGGCCGA210.13920190905475277No Hit
TGGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGG200.13257324671881213No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCGCTGACCCTACCGTGGTCGCC200.13257324671881213No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG200.13257324671881213No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG190.12594458438287154No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG190.12594458438287154No Hit
TAGAGTTTGATTATGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACAT190.12594458438287154No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG180.11931592204693094No Hit
GGTTACCTTGTTACGACTTAACCCCAATCATCCCTCCCACCTTAGGCCGA180.11931592204693094No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA180.11931592204693094No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACTGGTATTACCCTTAAGCGC180.11931592204693094No Hit
AGTTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC180.11931592204693094No Hit
AGGGTTTGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG180.11931592204693094No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTCACCCTAGGCCGA180.11931592204693094No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCGCTGATTCCACTGTGGACCAT180.11931592204693094No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC180.11931592204693094No Hit
GGTTACCTTGTTACGACTTCACCCCAATCACTATCCACACCTTAGATGCT180.11931592204693094No Hit
AGAGTTTGATTCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG170.11268725971099033No Hit
GGTTACCTTGTTACGACTTAACCCCAATCATGCCCCCCACCTTAGGCCGA170.11268725971099033No Hit
ACTGGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGC170.11268725971099033No Hit
AGAGTTTGATTCTGGCTCAGAGTGAACGCTGGCGGCGTGCCTAATACATG170.11268725971099033No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATCCGCCCTGCCTTCGGCAGC160.1060585973750497No Hit
TAGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGC160.1060585973750497No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCGGTATTACCCTAGTGCGC160.1060585973750497No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA160.1060585973750497No Hit
AGGGTTCGATTATGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG160.1060585973750497No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC160.1060585973750497No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG160.1060585973750497No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATTGGTTTTGCCTTAGGCGAT160.1060585973750497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)