FastQCFastQC Report
Thu 1 Sep 2022
EGAF00006623143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14177
Sequences flagged as poor quality0
Sequence length505-4193
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC8365.896875220427453No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC7665.403117725894053No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC4092.884954503773718No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC3822.6945051844536927No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2611.8410100867602455No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2571.8127953727869084No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2221.5659166255202088No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG2181.5377019115468717No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1741.2273400578401636No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1701.1991253438668266No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1591.1215348804401495No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1561.1003738449601468No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1531.0792128094801439No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA1451.0227833815334697No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC1390.980461310573464No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1220.8605487761867814No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA1030.7265288848134303No Hit
GGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA1020.719475206320096No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1020.719475206320096No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1010.7124215278267616No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG1000.7053678493334273No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC980.6912604923467588No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG930.6559920998800874No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG890.6277773859067504No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC870.6136700289200818No Hit
AGGGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG850.5995626719334133No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA840.592508993440079No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA830.5854553149467447No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA810.5713479579600762No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGA780.5501869224800734No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC770.543133243986739No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG740.5219722085067362No Hit
GGTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGCG720.5078648515200677No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG700.4937574945333992No Hit
AGAGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG700.4937574945333992No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG670.47259645905339637No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCGACTTTACCTTAGACGGC630.44438174508005923No Hit
AGGGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG600.4232207096000564No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG590.41616703110672215No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC580.4091133526133879No Hit
AGAGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG540.3808986386400508No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG510.359737603160048No Hit
AGTTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA500.35268392466671367No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG500.35268392466671367No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC500.35268392466671367No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA490.3456302461733794No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA490.3456302461733794No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG480.3385765676800451No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGCCGA470.33152288918671086No Hit
AGCTACCTTGTTACGACTTAGCCCCAGTCACCTGTATTACCCTAGGCCGA470.33152288918671086No Hit
AGAGTTCGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG450.3174155322000423No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG440.31036185370670805No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG440.31036185370670805No Hit
AGAGTTCGATTATGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG440.31036185370670805No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA430.3033081752133738No Hit
GGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC420.2962544967200395No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC410.28920081822670524No Hit
GGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC400.28214713973337097No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC390.2750934612400367No Hit
AGGGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG390.2750934612400367No Hit
AGGGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG370.2609861042533681No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG370.2609861042533681No Hit
GGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC360.25393242576003383No Hit
AGAGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG350.2468787472666996No Hit
AGGGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG340.23982506877336532No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG330.23277139028003105No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG330.23277139028003105No Hit
AGGGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG310.21866403329336248No Hit
GGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC310.21866403329336248No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG300.2116103548000282No Hit
CGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG290.20455667630669394No Hit
AGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA290.20455667630669394No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG290.20455667630669394No Hit
AGGGTTCGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG290.20455667630669394No Hit
AGTTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC280.19750299781335967No Hit
AGGGTTTGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG270.1904493193200254No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG270.1904493193200254No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG270.1904493193200254No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG270.1904493193200254No Hit
AGCTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC270.1904493193200254No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG250.17634196233335683No Hit
AGCTACCTTGTTACGACTTAGCCCCAATTACCAGTTTTGCCCTAGGTCGC250.17634196233335683No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG240.16928828384002256No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG240.16928828384002256No Hit
GGCTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC230.1622346053466883No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGC230.1622346053466883No Hit
AGGGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG220.15518092685335402No Hit
AGAGTTCGATTCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG220.15518092685335402No Hit
AGTTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC220.15518092685335402No Hit
AGCTACCTTGTTACGACTTCACCCCAGTCATCAGTCCTGCCTTCGGCAGC210.14812724836001975No Hit
AGGGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG210.14812724836001975No Hit
AGGGTTCGATTATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG210.14812724836001975No Hit
AGGGTTCGATTCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG210.14812724836001975No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT200.14107356986668548No Hit
AGAGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG200.14107356986668548No Hit
AGTTACCTTGTTACGACTTCACCCCAATCATCTATCCCACCTTAGGCGGC200.14107356986668548No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG200.14107356986668548No Hit
AGGGTTCGATTCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT200.14107356986668548No Hit
AGGGTTCGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG200.14107356986668548No Hit
CGGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG200.14107356986668548No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCGGTTTCACCCTAGGCCGA190.13401989137335119No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG190.13401989137335119No Hit
GGTTACCTTGTTACGACTTCGTCCCAATCGCCAATCCCACCTTCGACCAC190.13401989137335119No Hit
AGAGTTTGATCATGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG180.12696621288001692No Hit
GGTTACCTTGTTACGACTTAGCCCCAGTCACCGGTATTACCCTAGTGCGC180.12696621288001692No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCTCCCTGCCTTCGGCAGC180.12696621288001692No Hit
GGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGC170.11991253438668266No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGC170.11991253438668266No Hit
AGTTACCTTGTTACGACTTAGCCCCAATCACCGGTTTTACCCTAGGCCGA170.11991253438668266No Hit
AGGGTTCGATCATGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG170.11991253438668266No Hit
GCGGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAA170.11991253438668266No Hit
AGGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG170.11991253438668266No Hit
AGTTACCTTGTTACGACTTAGCGCTGCGACACGCTCGCGCGGCTACCTTG170.11991253438668266No Hit
AGGGTTCGATTCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG170.11991253438668266No Hit
GGCTACCTTGTTACGACTTAGCCCCAATCACCAGTTTTGCCCTAGGCCGT170.11991253438668266No Hit
AGGGTTTCGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT170.11991253438668266No Hit
AGAGTTCGATTCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG170.11991253438668266No Hit
GGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA160.11285885589334838No Hit
AGTTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA160.11285885589334838No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAGCCCTACCGTGGTAAGC160.11285885589334838No Hit
AGAGTTCGATTCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACAT160.11285885589334838No Hit
GGCTACCTTGTTACGACTTCACCCCAGTTATCCGCCCCGCCTTCGGCAGC160.11285885589334838No Hit
AGAGTTTGATCATGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG160.11285885589334838No Hit
GGCTACCTTGTTACGACTTCACCCCAATCGCTAATCACACCCTCGGAGCA160.11285885589334838No Hit
CAGTTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAG150.1058051774000141No Hit
AGGGTTTGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG150.1058051774000141No Hit
AGAGTTCGATCCTGGCTCAGGATGAACGCTAGCTATAGGCTTAACACATG150.1058051774000141No Hit
AGAGTTCGATTATGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG150.1058051774000141No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG150.1058051774000141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)