FastQCFastQC Report
Tue 9 Aug 2022
EGAF00006623177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153305878
Sequences flagged as poor quality0
Sequence length25-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC4467830.29143240026321754No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC3189320.20803638070550695No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCC3182520.2075928230227415No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC2796030.18238243937391624No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG2763360.18025140562451233No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2467490.1609520803892464No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC1875220.12231885851108723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)