FastQCFastQC Report
Wed 10 Aug 2022
EGAF00006623227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188271056
Sequences flagged as poor quality0
Sequence length25-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT4283780.2275325847218916No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG4153250.220599495654818No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC3672100.19504325720677956No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA3351880.17803480105832095No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG3286500.17456214831025327TruSeq Adapter, Index 7 (97% over 38bp)
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT2978910.1582245334620102No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC2756490.1464107154102328No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG2184600.11603483012279912No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC2129880.11312838230428791No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCT1896530.10073401829753374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)