FastQCFastQC Report
Tue 9 Aug 2022
EGAF00006623237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170092771
Sequences flagged as poor quality0
Sequence length25-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT7678490.4514295319464223No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG7487750.44021565149291386No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC6730290.3956834826331332No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA5931030.34869383132102655No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGAT5419160.31860025374035444No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC4343760.2553759324668772No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC3294360.19368018879532511No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG2741730.161190271866404TruSeq Adapter, Index 7 (97% over 36bp)
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC2437380.14329709520694445No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA1959510.1152024267980207No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG1882440.11067137003723691No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1863030.1095302280659535No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCC1745140.10259930447014706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)