FastQCFastQC Report
Tue 9 Aug 2022
EGAF00006623238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006623238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170092771
Sequences flagged as poor quality0
Sequence length25-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2823830.1660170496017141No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC2762320.16240078774423636Illumina Single End PCR Primer 1 (96% over 32bp)
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC2481550.14589391338683053No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2249680.13226194074996872No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT1965880.11557692831049239No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTC1723610.10133352463286051No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT1705270.10025528950904092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)