FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40782443
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1539800.37756443379323795Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA883020.21651964302383747No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC652070.15988988202594925Illumina Single End PCR Primer 1 (100% over 50bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC610090.14959623679238637No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT482960.11842350886139902No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC434570.10655810884110106No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)