FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57597291
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGG3361580.5836350879766203No Hit
GTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCG3133170.5439787089986576No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC2749470.47736099255084763No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC1721210.2988352351502087No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC1597670.2773863097137676No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG1534060.26634238752652445No Hit
GGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAG1269170.2203523773366355No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT1260190.2187932762323839No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC1259320.21864222746170472No Hit
CGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGG850740.14770486341102396No Hit
CCCAGCCGCGCACCCCGAGGAGCCCGGAGGCACCCCCGGGGGCGATTGAT710650.12338253894614593No Hit
TGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGG649350.11273967728794745No Hit
CAGCCGCGCACCCCGAGGAGCCCGGAGGCACCCCCGGGGGCGATTGATCG639810.11108334938877594No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN635570.11034720365581081No Hit
CTTAGGGGGACGGAGGGCCCCCGGCGGGCCCTGCGAGGGAACCCCCAGCC608800.10569941562008534No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC603630.1048018039598425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)