FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16907840
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTAT292095217.275725343982437TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTA1735831.0266420784677406TruSeq Adapter, Index 18 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTA668690.39549108579215325TruSeq Adapter, Index 18 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265350.15693902946798644No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTCGTATG222590.13164898650566836TruSeq Adapter, Index 18 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)