FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111998
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTAT308629733.87069444045093TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTA1918202.1051365463425253TruSeq Adapter, Index 16 (97% over 37bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTA685100.751865836669411TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATATCGTAT268960.29517126759685414TruSeq Adapter, Index 16 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTATG215220.23619408169316983TruSeq Adapter, Index 16 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGTATGCC104820.11503514377417554TruSeq Adapter, Index 16 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)