FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111998
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATGCTGGTGTAGATC1813951.9907269514326056Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1586011.740573252979204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTT1466221.6091092206122082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTTTTTTTTTTT505550.5548179444288728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTTTTTTTTTTT432760.474934257009275No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAGTTTTTTTTTTTTTTTTTTT273550.3002085821353341No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTGTTTTTTTTT211990.23264930479572096No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTGTTTTTTT201450.22108213807773003No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAATTTTTTTTTTTTTTTTTTTT165170.1812665015949301No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTGTTTTTTTTT123590.1356343581287002No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAATTTTTTTTTGTTTTTTTTT102070.11201714486767886No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGAAAAATTTTTTTTTTTTGTTTTTTT99430.10911986591744204No Hit
GGGGGGGGGGGGGGGGGGGGGGGGAAAAAAGTTTTTTTTTTTTTTTTTTT93130.10220590478619508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)