FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85653710
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTAT1330228415.530306860029764TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCAGAGATCTCGTAT3599730.42026550863938056TruSeq Adapter, Index 10 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTA3380010.3946133798524314TruSeq Adapter, Index 1 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTA3248410.3792491883889209TruSeq Adapter, Index 1 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCAGAGATCTCGTATG964630.11261975692588214TruSeq Adapter, Index 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)