FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75161531
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTAT61470818.1784935966778TruSeq Adapter, Index 7 (97% over 38bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTA1489570.19818249843793095TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATCTCGTA776250.10327756628587036TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTTCAATATCGTAT762780.10148542610181796TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)