FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130340304
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4273390.32786405040147826Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAG4119500.316057264988426No Hit
AGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCC2994520.22974628016825863No Hit
CTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGGATCTGTGACC2364700.18142507938296662No Hit
GAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCT1905120.1461650726240442No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1387560.10645671042780443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)