FastQCFastQC Report
Wed 16 Nov 2022
EGAF00006627412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006627412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143289592
Sequences flagged as poor quality0
Sequence length126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9850820.6874763102123984Illumina Single End PCR Primer 1 (100% over 50bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9337820.6516746868816543No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC2331310.1626991861348869No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC2134210.14894382559202207No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT1870970.13057263782285036No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA1495990.1044032563090835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)